Access your target proteins
on demand
Identifying and obtaining winning proteins is a challenge in the drug discovery workflow. eProtein Discovery™ empowers scientists to advance protein projects with confidence. It puts the latest technology on your bench to automate and optimise construct design, soluble expression and purification, providing you with protein to test in your applications.
Rapidly produce
protein
protein
Turn DNA into purified, soluble, active proteins in 48 hours — not weeks. Why wait when you don’t have to!
Multiplex constructs
and conditions
and conditions
Simultaneously screen 192 combinations of constructs and expression conditions.
Save time with automation
Integrate screening, characterisation and report generation. Time is on your side.
DOWNLOAD BROCHURES FOR MORE INFORMATION
Nuclera’s solution to protein discovery
eProtein Discovery System
Design and Optimisation Guide
Technology
Accelerating the journey to your protein with eProtein Discovery
There is an urgent, ever-expanding need for generating soluble and active proteins to be used for downstream high throughput screening in drug discovery. Obtaining soluble protein constructs using cell-based approaches can be time-consuming, costly and requires tremendous resources. We make this easy by combining unique cell-free technologies and novel digital microfluidics, turning protein access into a Pipette and Forget™ workflow that transforms weeks into days.
Nuclera’s eProtein Discovery is the only end-to-end protein prototyping system that accelerates construct design, expression, solubility characterisation and purification of target proteins in drug discovery programs.
The eProtein Discovery simple 3 step workflow:
1. Design & Prep
Design, order and prepare
DNA expression constructs
2. Load & Screen
Automated screens to determine your path
to soluble purifiable proteins
3. Scale up & Go
Scale up proteins at µg-
and mg- scale
De-risk your target proteins. Harness the power of cell-free protein synthesis and digital microfluidics
Explore more proteins faster and efficiently to test your hypotheses, and maximise your opportunity to identify and focus on targets that win. Screen 192 expression conditions simultaneously for optimal selection for scale-up. Rapidly identify the best recipes for making your protein, test expression feasibility earlier, and pursue targets with confidence. Fail fast, succeed faster!
eProtein Discovery: How the protein expression system works
Cell-free protein synthesis
By utilising cell extract derived reagents, proteins get synthesised in a droplet on our smart cartridge or in a tube. Screen 8 distinct expression conditions all at once. Each reaction includes purified cellular machinery, your carefully designed DNA construct and the cofactors, substrates, and energy systems needed to screen for optimal expressing conditions.
Protein detection
When you convert the cDNA for your protein of interest into an eProtein Discovery™ ready construct with our eGene™ Prep Kit, a sequence coding for a small peptide detection tag is installed. After expression of the eGene™ on the instrument, a detector protein is added that binds to the detection tag and becomes fluorescent. The optical system within the instrument measures fluorescence intensity and converts the reading into what matters to you – protein concentration.
Microfluidics-based smart cartridges
Based on rapid digital signals, and with the help of eProtein Discovery reagents, our software-controlled technology effectively turns DNA constructs into proteins for further characterisation. Covering 8 cell-free expression systems, our programmable microfluidics-based smart cartridges provide the features of complex cellular machinery with minimal time, effort, and resources.
Features
Rapidly produce protein
Our cell-free technology benefits from the advantages of complex cellular systems while bypassing lengthy, resource-consuming steps. Take high throughput bioreactors, liquid handlers and purification columns out of the equation. Optimal expression conditions identified in a day with our cell-free protein synthesis solution.
Multiplexed screening with multifluidic-based smart cartridges
eProtein Discovery operates with a digital microfluidics lab-on-a-cartridge designed to allow droplets of DNA to split, mix, and move within the smart cartridge. This allows the automation of separate screening workflows of expression, characterisation and purification all on the one run.
Explore multiple protein variations to speed up target ID
Simultaneously analyse 24 constructs, 8 expression conditions and purifiability of all combinations to find the winners for scale up. Screen protein isoforms, mutations, and more for simplified target identification.
Produce proteins on your terms and step forward with confidence
Assess the feasibility of your project by quickly testing candidate proteins. Purify µg to low mg quantities of your functional, fit-for-purpose protein yourself!
Information rich and intuitive data analysis
eProtein Discovery comes with software optimised for the instrument’s touchscreen interface. While providing clear instructions, the screen also allows you to monitor each step of your run in real-time. Following your run, receive an auto-generated report including solubility, soluble yield and purifiability through the eProtein report for pre-selecting the most promising candidate to scale up.
SYSTEM
eProtein Discovery system components
Instrument
The instrument orchestrates the rapid protein prototyping workflow enabling you to
Pipette and Forget™
Software
User-friendly software allows you to design your DNA constructs, customise your experimental conditions and perform data analysis
Cartridge
The smart cartridge automates multiplex screening of constructs and expression conditions
Reagents
An optimised suite of reagents for DNA preparation, protein expression screening, purifiability assessment and then off-cartridge scale up
DOWNLOAD BROCHURES FOR MORE INFORMATION
Nuclera’s solution to protein discovery
eProtein Discovery System
Functional membrane proteins in 48 hrs
Design and Optimisation Guide