REAGENTS FOR ENHANCED SEEDING AND ACCELERATED CELL GROWTH
Solentim’s technologies combine their technical expertise with an in-depth knowledge of cell creation, isolation, analysis and documentation to manage high value cells from discovery to production. Solentim’s enabling reagents and unparalleled domain expertise transform the speed and efficiency of clonally-derived Master Cell Bank development. Introducing Solentim’s MatriClone™, an imaging ready seeding matrix for iPSC cells, and InstiGRO™, a powerful growth supplement for CHO and HEK cells.
High viability single cell cloning and outgrowth
The Insti Reagents are cell growth supplements for CHO and HEK cells, designed to substantially improve cell growth under stressful conditions and help accelerate CLD workflows to maximise biotherapeutic production. MatriClone soluble matrices support iPSC growth, offering high levels of single cell survival and subsequent outgrowth which avoids the need for coating plates.
Designed for use with automated imaging
Designed to work with VIPS™ automation, Cell Metric imaging, ICON™ cell analysis, and manual cell growth processes, the Insti and MatriClone reagents transform workflows and greatly enhance the number of successful colonies per plate, conferring major growth and survival advantages.
Certified animal component-free
Solentim certify that no animal or human-derived materials are used in the manufacture of the raw material ingredients used in their Insti supplements and MatriClone matrix. Solentim’s reagents may contain materials produced by or extracted from plants, bacteria or yeast, or may contain recombinant human or animal proteins, provided they are produced in plants or micro-organisms.
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Next-gen cell supplements
In solution matrices for
iPSC cloning
The Solentim ecosystem
InstiGRO, InstiSHAKE™ AND InstiTHAW™
HIGH PERFORMANCE CELL GROWTH SUPPLEMENTS
In the search for high-value cells, growth conditions can be optimised to support early growth conditions. The Insti reagent range are proprietary animal component-free cell culture supplements for CHO and HEK cells which confer major growth and survival advantages within cell line development.
Designed to work with VIPS automation, Cell Metric imaging or manual cell practices, InstiGRO can transform workflows with greatly enhanced successful colonies per plate.
The Insti range of supplements are designed to enhance growth at different stages of cell line development for accelerated workflows:
- InstiGRO to assist with early cell growth and single cell survival
- InstiSHAKE as a follow on supplement in the transition to deep well plates
- InstiTHAW to protect cells during freezing and thawing
Optimising CHO cloning efficiency
A NEW STANDARD FOR iPSC SUPPORT WITH MatriClone iPSC MATRIX
High efficiency cloning of iPSCs
Soluble matrix for high efficiency, high viability iPSC single cell cloning and outgrowth
MatriClone is a new matrix designed to support iPSC growth in single seeding workflows while maintaining pluripotency. MatriClone is a defined matrix consisting of a recombinant peptide and is animal component-free. MatriClone is optimised for ‘in-solution’ seeding and optically suited for automated imaging. When used as part of the VIPS seeding protocol, MatriClone offers high levels of single cell survival and subsequent outgrowth, all in a simple, automated workflow which avoids the need for coating plates.
Designed for use with automated imaging
MatriClone offers ideal optical quality to contribute to high quality data when imaged by automated imaging platforms. This makes MatriClone ideally suited to workflows using post-seeding technologies including the Cell Metric® whole well imager.
Maintenance of phenotype, karyotype and pluripotency
Importantly, clones generated using the VIPS + MatriClone workflow have been shown by immunocytochemistry and karyotyping to maintain pluripotency after at least 10 passages and have retained their ability to successfully differentiate.
APPLICATIONS
Cell line development
Solentim’s InstiGRO and MatriClone cell culture supplements are specifically designed to work with VIPS automation, Cell Metric imaging or manual cell practices to transform cell line development workflows, with greatly enhanced successful colonies per plate.
Single cell cloning
of hiPSCs
CLD for protein therapies
Virus manufacturing for gene therapy
High efficiency, high viability single cell cloning of hiPSCs for applications including development of disease models and supporting GMP manufacture of cell therapies
Generate stable cell lines that create producing novel recombinant proteins such as monoclonal antibodies or therapeutic proteins for rare diseases
From the selection of high producer clones to the development of stable vector producer cell lines, explore our simplified workflows for gene therapy applications
SINGLE CELL CLONING OF hiPSCs
High efficiency, high viability single cell cloning of hiPSCs for applications including development of disease models and supporting GMP manufacture of cell therapies
CLD for Protein Therapies
Generate stable cell lines that create producing novel recombinant proteins such as monoclonal antibodies or therapeutic proteins for rare diseases
Virus manufacturing for gene therapy
From the selection of high producer clones to the development of stable vector producer cell lines, explore our simplified workflows for gene therapy applications
Vaccine production using mammalian cells
Engineered exosome producer cell lines
Cell engineering & precision gene editing
Isolate, screen and characterise attributes for clones producing novel recombinant sub-unit protein and monoclonal antibody vaccines
Establish stable producer cells for the production of engineered and cell-engineered exosomes
Optimise your drug discovery process using targeted gene editing to create stable cell lines as cellular reagents, for use in target validation, functional genomics and disease modelling
Vaccine production using mammalian cells
Isolate, screen and characterise attributes for clones producing novel recombinant sub-unit protein and monoclonal antibody vaccines
Engineered exosome producer cell lines
Establish stable producer cells for the production of engineered and cell-engineered exosomes
Cell engineering & precision gene editing
Optimise your drug discovery process using targeted gene editing to create stable cell lines as cellular reagents, for use in target validation, functional genomics and disease modelling
Resources
InstiGRO, InstiSHAKE and InstiTHAW
Advanced cell culture supplements for use with CHO and HEK cells
Culture healthier cells with InstiGRO proprietary animal component-free cell culture supplements for CHO and HEK cells.
Next-Generation cell supplements
MatriClone matrix for iPSC
Soluble matrix for high efficiency, high viability iPSC single cell
cloning and outgrowth
Support iPSC growth in single seeding workflows while maintaining pluripotency with MatriClone, an animal component-free matrix.
MatriClone
