SOLENTIM
VIPS PRO
VERIFIED IN-SITU PLATE SEEDING
Solentim’s unrivalled All-in-One VIPS PRO™ system uniquely combines single cell seeding and whole well imaging, providing up-front assurance of clonality, saving time and money
TAKE SINGLE CELL CLONING TO A NEW LEVEL OF QUALITY AND CONFIDENCE
The Solentim VIPS PRO™ system takes single cell cloning and image based clonality assurance to a whole new level of quality. This multi-tasking, compact instrument delivers a rock solid, ‘double lock’ solution for regulatory submission. With the VIPS PRO seeder, two-round methodologies are a thing of the past.
Elevating single cell seeding workflows
The VIPS PRO seeder accelerates projects. With VIPS PRO you get healthier cells, more confirmed single cells per plate and better outgrowth. High seeding efficiency for both 96 and 384-well plates, single round workflows for massively enhanced productivity and reduced timelines.
Delivering proof of monoclonality, not probability
With VIPS PRO, clonal assurance is based on evidence, unlike manual methods which use a repeated process to achieve probability of a single cell. VIPS PRO delivers evidence at multiple steps for complete certainty of single cell seeding — not in the nozzle, not in the tube, not in the air, but at the bottom of the well.
Highest assurance of clonality
Designed specifically for the task, Solentim technology is honed for single cell imaging in the droplet by way of z-stack imaging and in the whole well. With high clarity images, even when near the cell wall, VIPS PRO generates confidence for the lab and the regulator.
DOWNLOAD BROCHURES FOR MORE INFORMATION
The Solentim ecosystem
Workflows for IND success
Assurance of clonality
Understanding the ‘double lock’ of clonal assurance
Single cell seeding with in-well verification
Operating at a pressure of less than one psi, VIPS PRO gently dispenses cells in nanoliter droplets into a plate well. Crucially, the cell is imaged in the bottom of the dry well as evidence of seeding. Automated imaging in multiple tight z-stack layers of the whole droplet commences with intelligence-based image analysis confirming the existence of a single cell. The well is then filled automatically with media.
Outgrowth and clonal characterisation
During the clonal growth phase, VIPS PRO performs daily high-clarity whole well imaging, recording a timeline of well images and performing analysis to measure confluency. Successful clonal outgrowth can be traced back to a single cell to confirm monoclonality.
FEATURES
Designed to accelerate single-cell cloning workflows
Seeding for high clonal efficiency
For 96 and 384 well plates and quality single-project use consumables, we employ the simplest and safest method of single-cell dispensing, with no complex fluidics or mixed samples. VIPS PRO uses low pressure to seed cells in nanoliter droplets into empty wells.
In-line media dispensing
Immediately after confirmation of cell seeding, VIPS PRO automatically dispenses culture media into the well, protecting your high-value cell.
Seeding for high clonal efficiency
For 96 and 384 well plates and quality single-project use consumables, we employ the simplest and safest method of single-cell dispensing, with no complex fluidics or mixed samples. VIPS seeders use low pressure to seed cells in nanoliter droplets into empty wells.
In-line media dispensing
Immediately after confirmation of cell seeding, VIPS seeders automatically dispense culture media into the well, protecting your high-value cell.
In-well z-stack imaging
Immediately after seeding, the droplet is imaged at 20 different focal positions. This broad z-stack encompasses the optimal plane for image analysis enabling confident single cell detection.
Always in focus
VIPS PRO performs total plate laser focus scanning, adjusting for plate contours, to ensure an optimal focal position for each well.
In-well z-stack imaging
Immediately after seeding, the droplet is imaged at 20 different focal positions. This broad z-stack encompasses the optimal plane for image analysis enabling confident single cell detection.
Always in focus
VIPS seeders perform total plate laser focus scanning, adjusting for plate contours, to ensure an optimal focal position for each well.
Intelligent image analysis
Using advanced image analysis technology, z-stack images are processed to confirm the presence of a single cell.
QC verification process
To assist in the verification of your clonality workflow, the VIPS PRO offers a verification methodology option based on rare-event fluorescence imaging.
Intelligent Image Analysis
Immediately after seeding, the droplet is imaged at 20 different focal positions. This broad z-stack encompasses the optimal plane for image analysis enabling confident single cell detection.
QC verification process
To assist in the verification of your clonality workflow, the VIPS and VIPS PRO offer a verification methodology option based on rare-event fluorescence imaging.
SYSTEM
VIPS PRO
Elevating single cell seeding for your GMP-certified workflows
VIPS PRO’s powerful seeding technology gently and efficiently dispenses cells, reducing hands-on time and compresses timelines from months to days. Now with solutions to support GMP-certification, VIPS PRO provides certainty of clonality through superior imaging, advanced data management and single-use GMP-compliant consumables.
VIPS PRO System
DOWNLOAD BROCHURES FOR MORE INFORMATION
The Solentim ecosystem
Workflows for IND success
Assurance of clonality
APPLICATIONS
Cell line development
The Solentim VIPS PRO seeder accelerates single cell cloning workflows while also ensuring clonality to drive the discovery and development of antibody, cell & gene therapies and iPSC-based studies.
Single cell cloning
of hiPSCs
CLD for protein therapies
Virus manufacturing for gene therapy
High efficiency, high viability single cell cloning of hiPSCs for applications including development of disease models and supporting GMP manufacture of cell therapies
Generate stable cell lines that create producing novel recombinant proteins such as monoclonal antibodies or therapeutic proteins for rare diseases
From the selection of high producer clones to the development of stable vector producer cell lines, explore our simplified workflows for gene therapy applications
SINGLE CELL CLONING OF hiPSCs
High efficiency, high viability single cell cloning of hiPSCs for applications including development of disease models and supporting GMP manufacture of cell therapies
CLD FOR PROTEIN THERAPIES
Generate stable cell lines that create producing novel recombinant proteins such as monoclonal antibodies or therapeutic proteins for rare diseases
VIRUS MANUFACTURING FOR GENE THERAPY
From the selection of high producer clones to the development of stable vector producer cell lines, explore our simplified workflows for gene therapy applications
Vaccine production using mammalian cells
Engineered exosome producer cell lines
Cell engineering & precision gene editing
Isolate, screen and characterise attributes for clones producing novel recombinant sub-unit protein and monoclonal antibody vaccines
Establish stable producer cells for the production of engineered and cell-engineered exosomes
Optimise your drug discovery process using targeted gene editing to create stable cell lines as cellular reagents, for use in target validation, functional genomics and disease modelling
VACCINE PRODUCTION USING MAMMALIAN CELLS
Isolate, screen and characterise attributes for clones producing novel recombinant sub-unit protein and monoclonal antibody vaccines
ENGINEERED EXOSOME PRODUCER CELL LINES
Establish stable producer cells for the production of engineered and cell-engineered exosomes
CELL ENGINEERING & PRECISION GENE EDITING
Optimise your drug discovery process using targeted gene editing to create stable cell lines as cellular reagents, for use in target validation, functional genomics and disease modelling
ABOUT SOLVE SCIENTIFIC
Solve Scientific caters to the Australian and New Zealand scientific market with high-quality product and innovative technologies. In close partnership with our suppliers our role is delivering premium local support in enabling our customer’s results.
APPLICATIONS
- LIQUID HANDLING
- SAMPLE PREPARATION
- PURIFICATION
- CELL LINE DEVELOPMENT
- GENE THERAPY
- NATURAL PRODUCTS